Combined effect of menaquinone 4 and coenzyme Q10 evaluation for the cryopreservation of ovine semen
DOI:
https://doi.org/10.71112/c9qe0m86Keywords:
L-carnitine, molecular weight, sperm membrane, combined effectAbstract
This experimental study analyzed the impact of the combined use of two antioxidants, menaquinone-4 and coenzyme Q10, on the quality of ovine semen subjected to cryopreservation, comparing them with the combined effect of each molecule together with L-carnitine and their individual use. This model allowed the evaluation of possible redox effects of the combinations and their saturation in the medium due to their high molecular weight. The effects of these six combinations were assessed in eight ejaculates, analyzing sperm motility, membrane integrity, and cell viability through eosin-nigrosin staining, propidium iodide, rhodamine, and motility evaluation using the CASA system. The results demonstrated a negative effect of the molecular combinations on sperm quality, attributable to medium saturation and increased ionic load, which generated damage to the sperm membrane. It was concluded that limited use of antioxidants in semen extenders is recommended..
Downloads
References
Abecia, J. A., Carvajal-Serna, M., Casao, A., Palacios, C., Pulinas, L., Keller, M., Chemineau, P., & Delgadillo, J. A. (2020). The continuous presence of ewes in estrus in spring influences testicular volume, testicular echogenicity and testosterone concentration, but not LH pulsatility in rams. Animal, 14(12), 2554–2561. https://doi.org/10.1017/S1751731120001330
Adami, L., Maciel Júnior, V., & De Agostini Losano, J. (2022). Review on the role of antioxidant supplementation against oxidative stress: A human and animal approach to male fertility. Research, Society and Development, 11(1). https://doi.org/10.33448/rsd-v11i1.25191
Aguirre-Riofrio, E. (2023). Estudio de la actividad reproductiva. Cedamaz, 13(2), 190–194. https://doi.org/10.54753/cedamaz.v13i2.2035
Aitken, R. J., & Drevet, J. R. (2020). The importance of oxidative stress in determining the functionality of mammalian spermatozoa: A two-edged sword. Antioxidants, 9(2), 111. https://doi.org/10.3390/antiox9020111
Bansal, A. K., & Bilaspuri, G. S. (2011). Impacts of oxidative stress and antioxidants on semen functions. Veterinary Medicine International, 2011, 686137. https://doi.org/10.4061/2011/686137
Chavoshi Nezhad, N., Vahabzadeh, Z., Allahveisie, A., Rahmani, K., Raoofi, A., & Rezaie, M. J. (2021). The effect of L-carnitine and coenzyme Q10 on sperm motility, DNA fragmentation, chromatin structure and oxygen free radicals during, before and after freezing in oligospermia men. Urology Journal, 18(3), 330–336. https://doi.org/10.22037/uj.v16i7.6400
Cofré, E., Peralta, O. A., Raggi, A., De Los Reyes, M., Sales, F., González-Bulnes, A., & Parraguez, V. H. (2018). Ram semen deterioration by short-term exposure to high altitude is prevented by improvement of antioxidant status. Animal, 12(5), 1007–1014. https://doi.org/10.1017/S1751731117002452
Farfán, K., Alvarado, J., & Moscoso, P. (2025). Determinación del efecto post congelación de microdosis de menaquinona-4 en semen ovino. MQRInvestigar, 9(3), 1–19. https://doi.org/10.56048/MQR20225.9.3.2025.e903
Gautier, C., & Aurich, C. (2022). Fine feathers make fine birds – The mammalian sperm plasma membrane lipid composition and effects on assisted reproduction. Animal Reproduction Science, 246. https://doi.org/10.1016/j.anireprosci.2021.106884
Guamarrigra Sánchez, I. C., Alvarado Sáciense, J. C., Moscoso Piedra, A. L., & Maldonado Cornejo, M. E. (2025). Evaluación del efecto combinado de coenzima Q10 y L-carnitina sobre la crioconservación de semen ovino. ASCE, 4(3), 1793–1811. https://doi.org/10.70577/ASCE/1793.1811/2025
Guiracocha-Viñanzaca, I. M., Moscoso-Piedra, A., & Alvarado-Alvarado, J. C. (2025). Efecto de microdosis de coenzima Q10 en la refrigeración de semen ovino previo a la congelación. MQRInvestigar, 9(1). https://doi.org/10.56048/MQR20225.9.1.2025.e114
Iliceto, M., Andersen, J., Haug, M., Haugen, T., & Witczack, O. (2024). Association of endogenous seminal L-carnitine levels with post-thaw semen parameters in humans. Andrology, 2024, 4327010. https://doi.org/10.1155/2024/4327010
Juliani, G., & Henry, M. (2008). Efeito do glicerol, etilenoglicol, acetamida e leite desnatado na criopreservação de espermatozóides eqüinos. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 60, 1103–1109.
Kowalczyk, A. (2022). The role of the natural antioxidant mechanism in sperm cells. Reproductive Sciences, 29, 1387–1394. https://doi.org/10.1007/s43032-021-00795-w
Larbi, A., Anass, B., Maia, M. D., Boubker, N., & Bouchra, E. (2018). Supplementation of ram semen extender to improve seminal quality and fertility rate. Animal Reproduction Science, 192, 6–17. https://doi.org/10.1016/j.anireprosci.2018.03.019
Lema Guamán, M. F., Maldonado Cornejo, M. E., Alvarado Booker, J. C., & Moscoso Piedra, A. L. (2025). Evaluación del efecto combinado de la menaquinona-4 y L-carnitina sobre la crioconservación de semen ovino. ASCE Magazine, 4(3), 1906–1926.
Loja, E., Maldonado, M., Alvarado, J., & Moscoso, A. (2025). Efecto de la concentración de la coenzima Q10 en semen ovino post congelación. Revista Espamciencia, 16(2), 30–36.
Mehdipour, M., Mohammadi, H., & Salih, S. (2025). Mitochondrial specific antioxidant MitoPBN mitigates oxidative stress and improves mitochondrial function in cryopreserved ram sperm. Scientific Reports. https://doi.org/10.1038/s41598-025-95820-2
Moreira, F. M., Martins, A., Oliveira, P., Alves, M., & Pereira, M. (2023). L-carnitine and male fertility: Is supplementation beneficial? Journal of Clinical Medicine, 12(18), 5796. https://doi.org/10.3390/jcm12185796
Nogueira, B. G., Pereira, R. R., Bitencourt, J. L., Milan, B. R. W. V. A., Junior, M. V., et al. (2022). Coenzyme Q10 and melatonin protect cryopreserved equine sperm against lipoperoxidation. Animal Reproduction Science, 243. https://doi.org/10.1016/j.anireprosci.2022.107027
Puerta, M., Henao-Salazar, L., Vélez, I., León, S., Rojano, B., Restrepo, G., & Úsuga, A. (2024). Dietary antioxidant supplementation improves the in vitro quality and antioxidant capacity of Colombian Creole stallion semen. Czech Journal of Animal Science, 69(11), 450–461. https://doi.org/10.17221/98/2024-CJAS
Reyes-González, E. A., Centurión-Castro, F., Uc Espinosa, N., Ek-Mex, J. E., Peralta-Torres, J. A., & Segura Correa, J. C. (2022). Effect of Ley-Leygo and Triladyl extenders and two equilibration times on the freezing of boar semen. Revista de Investigaciones Veterinarias del Perú, 33(6). https://doi.org/10.15381/rivep.v33i6.22758
Reza, M., Nader, A., & Mohsen, S. (2021). Effects of freezing extender supplementation with mitochondria-targeted antioxidant Mito-TEMPO on frozen-thawed rooster semen quality and reproductive performance. Animal Reproduction Science, 225. https://doi.org/10.1016/j.anireprosci.2020.106671
Rodríguez, S. (2024). Evaluación de la calidad espermática de semen porcino criopreservado en diferentes niveles de coenzima Q10 [Tesis de grado, Escuela Superior Politécnica de Chimborazo]. http://dspace.espoch.edu.ec/handle/123456789/23037
Sadler, R., Shoveller, A., Shandilya, R., Charchoglyan, A., & Wagter-Lesperance, L. (2024). Más allá de la cascada de la coagulación: La vitamina K y su impacto multifacético en la salud humana y de los animales domésticos. Current Issues in Molecular Biology, 7001–7031.
Uribe Valderrama, R., Arango Rodríguez, E., Rendón Álvarez, L., & Naranjo, M. A. (2011). Evaluation of canine semen that has gone through a freezing and thawing process with different glycerol concentrations as a cryoprotector. CES Medicina Veterinaria y Zootecnia, 6(1).
Valdez-Pinargote, Moscoso-Piedra, A., & Maldonado-Cornejo, M. (2024). Efecto de la concentración de la L-carnitina en congelación del semen ovino. Journal Scientific, 8(4). https://doi.org/10.56048/MQR20225.8.4.2024.7324-7341
Vicente-Fiel, S., Palacín, I., Santolaria, P., Fantova, E., Quintín-Casorrán, F. J., Sevilla-Mur, E., & Yániz, J. L. (2014). In vitro assessment of sperm quality from rams of high and low field fertility. Animal Reproduction Science, 15–20. https://doi.org/10.1016/j.anireprosci.2014.02.005
Wang, W., Fang, H., Groom, L., Cheng, A., Zhang, W., Liu, J., & Cheng, H. (2008). Superoxide flashes in single mitochondria. Cell, 134(2), 279–290.
Watson, P. F. (2000). The causes of reduced fertility with cryopreserved semen. Animal Reproduction Science, 60–61, 481–492. https://doi.org/10.1016/S0378-4320(00)00099-3
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Dayana Patricia Castro-Sanchez, Manuel Esteban Maldonado-Cornejo, Andrés Leonardo Moscoso-Piedra (Autor/a)
This work is licensed under a Creative Commons Attribution 4.0 International License.
